Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 581,575:1 +G 100% coding (1613/2277 nt) clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE581,5751.G85.7% 15.3 / ‑2.1 7coding (1613/2277 nt)clpAATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (6/0);  total (7/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GTCAGCCGTCTTATTGGTGCGCCTCC‑GGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAG  >  minE/581550‑581627
                          |                                                    
gTCAGCCGTCTTATTGGTGCGCCTCC‑GGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATg             >  1:197528/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:1241204/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:133512/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:1568282/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:1620468/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:716185/1‑67 (MQ=255)
            aTTGGTGCGCCTCCGGGGATACGTTGGGTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAg  >  1:1702458/1‑67 (MQ=255)
                          |                                                    
GTCAGCCGTCTTATTGGTGCGCCTCC‑GGGATACGTTGGTTTTGATCAGGGCGGTTTGCTGACTGATGCGGTCATCAAG  >  minE/581550‑581627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑