Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	983,543:1	+A	100%	intergenic (+455/‑113)	ydfC → / → dicB	hypothetical protein/cell division inhibition protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	983,543	1	.	A	100.0%	40.2 / NA	13	intergenic (+455/‑113)	ydfC/dicB	hypothetical protein/cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/13);  total (0/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑AAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTG  >  minE/983481‑983587                                                                |                                             cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGTAACAGTTAAAACC‑aaaa                                          <  1:89729/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:305160/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:93096/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:890699/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:839342/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:782065/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:626054/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:602285/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:354590/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1020905/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:2186778/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1113284/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1966601/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1276348/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1554719/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:137715/67‑1 (MQ=255) cTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:1984770/67‑1 (MQ=255)  tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:468021/66‑1 (MQ=255)  tttttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑aaaa                                          <  1:721662/66‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:1486392/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:1243904/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:1348123/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:713154/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:711689/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:1383482/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:590510/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:432532/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:1587153/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:2307094/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:217657/67‑1 (MQ=255)                                          cgcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:2070165/67‑1 (MQ=255)                                           gcACGCGGAACAGTTAAAACCAAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTg  <  1:895908/66‑1 (MQ=255)                                                                |                                             CTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACC‑AAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTG  >  minE/983481‑983587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑