| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,910:1 | +C | 100% | intergenic (‑31/+208) | ygdL ← / ← mltA | conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,910 | 1 | . | C | 86.7% | 40.0 / 1.3 | 15 | intergenic (‑31/+208) | ygdL/mltA | conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (0/13); total (0/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ACTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTAT‑CCCTCACCCTAAACGCATAAATGCCTGATGCGCTACGCTTATC > minE/1892862‑1892953 | aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1045842/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1090678/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1194886/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1672223/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1786644/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:1844206/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:2122035/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:2155933/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:2172322/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:21816/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:666037/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:818653/67‑1 (MQ=255)aCTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTATCCCCTCACCCTAAACGCa < 1:871048/67‑1 (MQ=255) cacaaTTAAAAAGGGCGAGGGTAT‑CCCTCACCCTAAACGCATAAATGCCTGATGCGCTACGCTTATc < 1:1708965/64‑1 (MQ=255) cAGAATTAAAAAGGGCGAGGTTAT‑CCCTCACCCTAAACGCATAAATGCCTGATGCGCTACGCTTATc < 1:139818/67‑1 (MQ=255) | ACTAATTACCACAGACATAGCACCTCAGAATTAAAAAGGGCGAGGTTAT‑CCCTCACCCTAAACGCATAAATGCCTGATGCGCTACGCTTATC > minE/1892862‑1892953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |