Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	minE	516424	516479	56	27 [0]	[0] 20	bioB	biotin synthase

GTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAC  >  minE/516357‑516423                                                                   | gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:2097384/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:982505/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:945360/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:873381/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:866619/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:802830/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:727544/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:590762/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:548905/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:305782/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:301480/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:2247944/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:2212420/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1115026/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:2088136/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1851036/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1801302/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1700268/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:154153/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1525890/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1501152/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1326074/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1286884/67‑1 (MQ=255) gTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1130807/67‑1 (MQ=255)   cTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:683787/65‑1 (MQ=255)    tGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1481743/64‑1 (MQ=255)                  tgtgGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAc  <  1:1587999/50‑1 (MQ=255)                                                                   | GTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAAC  >  minE/516357‑516423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑