Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	minE	2525199	2525250	52	12 [0]	[0] 18	rph	Mutation in protein compared to W3110: p.Val138Ala; p.Val172Ile; p.Ile216Thr; p.Glu224_Glyfs*16 ECK3633:JW3618:b3643; ribonuclease PH

CACACCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGC  >  minE/2525135‑2525198                                                                | cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACTCACAATGGAAATCCAGc  <  1:2009654/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:1358373/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:16121/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:2286696/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:314268/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:387229/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:40928/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:42026/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:557519/64‑1 (MQ=255) cacaCCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:986734/64‑1 (MQ=255)  acacCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:2173428/63‑1 (MQ=255)        gTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGc  <  1:614468/57‑1 (MQ=255)                                                                | CACACCCGTAACGCTCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGC  >  minE/2525135‑2525198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑