| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 11,695 | G→A | 100% | A31V (GCC→GTC) | yaaI ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 11,695 | 0 | G | A | 92.4% | 313.5 / 11.3 | 105 | A31V (GCC→GTC) | yaaI | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (0/97); total (8/97) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 106 total aligned reads displayed.
ACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGG > minE/11632‑11745 | acaATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGgcg > 1:223915/1‑66 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:1892038/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:288372/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:1595403/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:254465/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:1848597/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:2154627/1‑67 (MQ=255) tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGttt > 1:890995/1‑67 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2263390/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:204386/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2069971/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:282398/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:263752/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2139480/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:251838/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:250627/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:243137/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2308913/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2271050/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2190968/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2262419/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2053167/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2259528/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2227323/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2197601/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2196512/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2196269/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:657706/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:95137/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:934629/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:929070/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:878823/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:866016/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:858831/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:792868/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:792499/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:749698/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:71244/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:659141/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:292814/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:620292/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:583586/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:539349/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:488991/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:443782/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:428149/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:359867/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:334707/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:333442/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:332181/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:316525/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1311492/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1553038/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1538014/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1527279/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1458225/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1438361/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1415726/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1411746/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1406921/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1375753/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1368095/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:132969/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1558980/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1273105/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1233483/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1232596/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:110159/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1098567/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1084439/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1077278/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1071901/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1029053/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1026407/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1026307/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1818502/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:102527/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:200526/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1999613/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1994673/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1964242/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:194978/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1941370/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1925510/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1910809/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1905827/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1904657/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:2045580/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1809547/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1794927/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1794590/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:171119/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:171098/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1673577/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1653454/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1651867/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1634603/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1622979/66‑1 (MQ=255) tcgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:1614417/66‑1 (MQ=255) cgcgGCATTGCAATGACGCCGAGGATTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAgg < 1:759506/66‑1 (MQ=255) | ACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGG > minE/11632‑11745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |