Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	226,273	T→A	100%	R123R (CGT→CGA)	frsA →	hydrolase, binds to enzyme IIA(Glc)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	226,273	0	T	A	100.0%	192.2 / NA	61	R123R (CGT→CGA)	frsA	hydrolase, binds to enzyme IIA(Glc)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (61/0);  total (61/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTG  >  minE/226212‑226278                                                              |      cccATGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:297278/1‑67 (MQ=255) cccATGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:523/1‑67 (MQ=255) cccAGGCGATGGTGTTGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1102784/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGGTCCGCAACTCAGCGGTCGACACTg  >  1:1577927/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGacac    >  1:305339/1‑65 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACt   >  1:746796/1‑66 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACt   >  1:1674193/1‑66 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2147901/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2260478/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2242726/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:221455/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2174526/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:309592/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2137338/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2074869/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2067805/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2058345/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2030164/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2021048/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2263164/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1988761/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:325360/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:336963/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:424264/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:528130/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:538486/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:66523/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:725079/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:737919/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:746867/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:831206/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:859066/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:886190/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1385114/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1040854/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1059241/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1072288/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1136690/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1152804/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1162926/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:117196/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1306367/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1307766/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1314620/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1320269/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1325771/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1371099/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1382552/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:2007120/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1429579/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1466799/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1490906/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1514743/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1589182/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1595123/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1675525/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:171355/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1818313/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1880032/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1962411/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1971305/1‑67 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTg  >  1:1039351/1‑67 (MQ=255)                                                              |      CCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTG  >  minE/226212‑226278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑