| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,004,302 | G→A | 100% | S483F (TCT→TTT) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,004,302 | 0 | G | A | 95.9% | 72.7 / ‑3.8 | 24 | S483F (TCT→TTT) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (0/23); total (1/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCG > minE/2004238‑2004353 | ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1050206/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:452573/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:501040/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:54612/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:36546/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2277618/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2231393/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2118754/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2038143/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2014924/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1891603/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1861324/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1788786/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:716486/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:739087/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1708773/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1359767/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1288814/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1286299/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:829500/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1275880/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1182671/67‑1 (MQ=255)ccAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:943265/67‑1 (MQ=255) cAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:767102/66‑1 (MQ=255) cAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:961812/66‑1 (MQ=255) cAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:606708/66‑1 (MQ=255) tttaTGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1212929/59‑1 (MQ=255) cTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:2285193/46‑1 (MQ=255) gTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgaaag < 1:1501174/43‑1 (MQ=255) ataaCCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCg > 1:481311/1‑67 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:913081/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:563701/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:573687/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:664355/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:104841/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:966843/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:71328/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:464150/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:391018/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:369247/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:241622/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1979603/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1864782/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:180036/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1775085/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1753465/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1725295/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1281199/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:124463/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1218999/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1164724/52‑1 (MQ=255) aCCATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:1143473/52‑1 (MQ=255) ccATCCAGTGAAAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCtt < 1:2060463/51‑1 (MQ=255) | CCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCG > minE/2004238‑2004353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |