| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,070,862 | G→T | 100% | R228L (CGC→CTC) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,070,862 | 0 | G | T | 75.5% | 69.9 / 34.9 | 49 | R228L (CGC→CTC) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (12/0); new base T (0/37); total (12/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAA > minE/2070796‑2070886 | gCAAACCGTTTATGCCGTTACTGCCGGGCTTCGGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1695816/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTTATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1657444/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1082522/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:663569/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:625355/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:51375/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:472340/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1996317/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1940500/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1675723/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1559886/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1553879/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1419634/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1406760/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1309433/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1165951/1‑67 (MQ=255)gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATgcg > 1:1166179/1‑67 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCTTCATGTACCGTTTGTCAATATCGAAGCCATGCTCACGGcgc < 1:542017/67‑3 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACTGctc < 1:607027/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1998149/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:212515/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:204115/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:2115943/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:2153514/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:2193832/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:266169/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:315874/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1201359/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:495766/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:554033/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1038677/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:68117/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:87131/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:92339/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:951814/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:965900/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:19803/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1423737/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1399540/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1573570/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1576088/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1617391/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1390205/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1369961/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:16866/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1317355/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1753042/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1812812/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1268184/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1868025/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:182089/67‑1 (MQ=255) tttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1856558/67‑1 (MQ=255) ttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:1641125/66‑1 (MQ=255) ttATGCCACTGCTGCCAGGGTTTCGTCATGTACCGTTTGGCAATATCGAAGCCATGCTCACGGctc < 1:2156382/66‑1 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:237429/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:1834486/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:630661/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:269248/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:169685/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:708122/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:752690/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:839526/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:1411295/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:1871972/1‑50 (MQ=255) ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:306304/1‑50 (MQ=255) ccGTATGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCaaaaa > 1:294742/1‑50 (MQ=255) | GCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAA > minE/2070796‑2070886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |