| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,136,124 | G→A | 100% | G69G (GGC→GGT) | pnp ← | polynucleotide phosphorylase/polyadenylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,136,124 | 0 | G | A | 80.0% | 198.3 / 42.7 | 95 | G69G (GGC→GGT) | pnp | polynucleotide phosphorylase/polyadenylase |
| Reads supporting (aligned to +/- strand): ref base G (1/18); new base A (0/76); total (1/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGGTCAGTGGGAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCCAACAACGGTAACGAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGC > minE/2136079‑2136183 | aaCGGTCAGTGGGAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCCAACAACGGTAACGAATAcc < 1:1963306/67‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:33901/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:308192/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:288733/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:1337004/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:1814814/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:729571/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:311895/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:1250295/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:313428/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:148358/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:186623/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:220996/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:344045/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:48852/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:443370/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:1652903/39‑1 (MQ=255) gggAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCca < 1:91344/39‑1 (MQ=255) gCTTTTTTCTGGCCAACAACGGTAACGAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGc > 1:142179/1‑65 (MQ=255) tttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1051375/66‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:246251/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:372767/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:537492/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2315864/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2269899/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2225609/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2192971/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2188338/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2146366/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2133927/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2054446/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:767067/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:944000/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:885352/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:876159/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:875489/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:871073/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:854060/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:79943/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:77376/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:770129/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:769432/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:417402/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:761634/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:635475/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:634292/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:61076/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:605179/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:581890/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:56947/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1031010/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:42573/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1202286/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1540405/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1491962/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1477711/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1442376/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1426358/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1396574/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1331202/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:129031/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1267284/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1233189/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2014483/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1191901/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1190582/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1188435/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1148455/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1137174/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1113199/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:110129/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:106184/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1060956/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1570186/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:2028059/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:199564/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1974420/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1881430/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1866955/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1840942/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1837848/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1828605/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1812152/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1803209/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1802160/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1797152/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1733728/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:167051/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1652886/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1579993/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1574573/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1558987/65‑1 (MQ=255) ttCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCACTAGc < 1:1899959/65‑1 (MQ=255) tCTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1852711/64‑1 (MQ=255) cTGACCAACAACGGTAACAAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGc < 1:1013578/63‑1 (MQ=255) | AACGGTCAGTGGGAAGAAGTCCTGACCTGGTTTGGCTTTTTTCTGGCCAACAACGGTAACGAATACCGCGGTGTCATCCATGCTAACCATAACAGCGGCAGTAGC > minE/2136079‑2136183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |