| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,148,960 | T→A | 100% | A68A (GCA→GCT) | glmM ← | phosphoglucosamine mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,148,960 | 0 | T | A | 99.1% | 366.9 / ‑4.5 | 105 | A68A (GCA→GCT) | glmM | phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (0/104); total (0/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 105 total aligned reads displayed.
CCACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTG > minE/2148911‑2149014 | ccACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGGGCGGAAAGGCCCGCTGCCGCCAGACCCGAtt < 1:39459/66‑1 (MQ=255) ccGGTGTTGTCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:156642/67‑1 (MQ=255) ccGGTGTTGGCATCGTACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2175867/67‑1 (MQ=255) ccGGTGTTGGCATCGGCCCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2067071/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCTCTTCCAGTGc < 1:2257758/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCTCTTCCAGTGc < 1:2255144/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1785953/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1012006/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1816748/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1828994/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1907493/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1920758/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1936157/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2033398/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2068817/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2074603/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:221003/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:443772/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:558740/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:594793/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:605525/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:637516/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:658424/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:737137/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:775455/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:838649/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:846933/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:902597/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1586476/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1069417/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1076534/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1573840/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1564192/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1133684/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1159973/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1200534/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1524970/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1306052/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1389530/67‑1 (MQ=255) ccGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1379299/67‑1 (MQ=255) ccGGTCTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:30010/67‑1 (MQ=255) cGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1608425/66‑1 (MQ=255) cGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:917980/66‑1 (MQ=255) cGGTGTTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:2262165/66‑1 (MQ=255) ggTGTTGGCATCGGACCAGTGAAGAGTGCTGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1325877/65‑1 (MQ=255) gtgtTGGCATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:1014941/64‑1 (MQ=255) cATCGGACCAGTGAAGAGTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:467354/57‑1 (MQ=255) gTGCGGAAAGGCCCGCAGCCGCCAGACCCGCTTCCAGTGc < 1:591952/40‑1 (MQ=255) atcaGGCCCGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:119468/63‑1 (MQ=255) gCCCGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:788942/61‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCGAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:363484/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATTTAGCCAGAAATACGCgtg < 1:2029341/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:517719/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:505126/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:638301/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:538837/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1852235/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:489389/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1557549/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:110548/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:610035/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:612571/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1078055/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:4238/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1766115/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1041689/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:812764/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:812998/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:837637/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1033456/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:857176/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1001189/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:908826/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1628159/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2210422/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1989019/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2020723/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2022204/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:193701/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1323350/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1318588/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2088351/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2145434/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:215708/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1923652/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1279154/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1189253/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2220544/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1265966/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1537483/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1905550/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:2314800/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:236389/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:300074/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1852330/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:389581/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:39928/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1975086/59‑1 (MQ=255) ccGCAGCCGCCAGACCCGCTTCAAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:1693711/59‑1 (MQ=255) ccGCAGCCGCCAGACACGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg < 1:421779/59‑1 (MQ=255) | CCACGGCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTG > minE/2148911‑2149014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |