Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,262,618	C→T	100%	intergenic (‑227/+186)	rplJ ← / ← rplA	50S ribosomal subunit protein L10/50S ribosomal subunit protein L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,262,618	0	C	T	100.0%	68.9 / NA	21	intergenic (‑227/+186)	rplJ/rplA	50S ribosomal subunit protein L10/50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/0);  total (21/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTT  >  minE/2262579‑2262645                                        |                            ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAg                >  1:1721471/1‑53 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1941693/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:955944/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:903541/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:649392/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:584488/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:464818/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:329500/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:283883/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:2201791/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:2090661/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:2043656/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1013475/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1858631/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1653505/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1515999/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:148914/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1419865/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:141265/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt  >  1:1031542/1‑67 (MQ=255) ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGt   >  1:1020168/1‑66 (MQ=255)                                        |                            CCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTT  >  minE/2262579‑2262645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑