Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,295,315	G→A	100%	G161G (GGC→GGT)	yijE ←	predicted permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,295,315	0	G	A	100.0%	232.8 / NA	72	G161G (GGC→GGT)	yijE	predicted permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (72/0);  total (72/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACTGACGCCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCT  >  minE/2295308‑2295373        |                                                           aaCTCACACCGGAGAGGATTGCCAGCATGGCACttt                                >  1:2234256/1‑36 (MQ=37) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGTAATCGAGTTGCCACGGCt  >  1:748752/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGc   >  1:955914/1‑65 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGc   >  1:1598050/1‑65 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:310831/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:462207/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:457640/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:43607/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:358007/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:35393/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:333869/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:316643/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:557596/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:286768/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:257549/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2268789/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2228033/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2227827/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2150168/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2113666/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:769759/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:968143/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:962260/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:946318/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:874209/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:85681/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:833125/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:776709/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:772228/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:502100/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:751564/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:715542/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:702372/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:630307/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:573989/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2055288/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:545788/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1440588/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1622113/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1621052/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1611512/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1534438/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1533481/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1509185/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1477016/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1452525/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1662029/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1430304/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1420839/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:141732/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1332081/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1098055/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1084141/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1069639/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:180468/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2017302/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:2003469/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1951477/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:194742/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1922428/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1918069/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1851899/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1015669/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1797412/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1780235/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1773980/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1735680/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1727714/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1720397/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1705321/1‑66 (MQ=255) aaCTCACACCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCt  >  1:1662563/1‑66 (MQ=255) aaCTCACACCGGAGAGCATTGCCAGCATGGCACTTTTCATCGAAGACAAATCGAGTTGCCACGGCt  >  1:1079513/1‑66 (MQ=255)        |                                                           AACTGACGCCGGAGAGGATTGCCAGCATGGCACTTTTCATCGAAGAGAAATCGAGTTGCCACGGCT  >  minE/2295308‑2295373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑