Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,415,878	G→T	100%	noncoding (1/77 nt)	proM ←	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,415,878	0	G	T	100.0%	65.0 / NA	20	noncoding (1/77 nt)	proM	tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/0);  total (20/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGC  >  minE/2415821‑2415887                                                          |          cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGGGCTACTCGCCTAAATACTGc  >  1:1475412/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATAc     >  1:1992606/1‑64 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1979918/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:714068/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:705249/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:64844/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:621109/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:595464/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:2249025/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:2060658/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1997630/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1182558/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1977688/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1868929/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1766305/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1564042/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1433517/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1418169/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1400449/1‑67 (MQ=255) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCTAAATACTGc  >  1:1319710/1‑67 (MQ=255)                                                          |          CGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGC  >  minE/2415821‑2415887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑