| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,422,871 | T→C | 100% | L199L (TTA→TTG) | rffA ← | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,422,871 | 0 | T | C | 79.0% | 151.4 / 35.2 | 76 | L199L (TTA→TTG) | rffA | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Reads supporting (aligned to +/- strand): ref base T (14/2); new base C (60/0); total (74/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGAACTGGCTGCGGTTAGTGCCCTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTAATCAGCGTC > minE/2422818‑2422895 | aagatCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:297779/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTAt > 1:91896/1‑64 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:2055253/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:748338/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:1245912/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:1675284/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:52677/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:1456121/1‑65 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2199430/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1997540/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:271705/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:243626/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2306944/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2265080/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2257210/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1108590/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2153716/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2135223/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2108653/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2013772/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:2003092/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:327596/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:364405/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:446049/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:455076/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:554367/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:564419/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:572170/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:62691/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:723274/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:815267/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:838106/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:849627/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:861794/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:909305/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1934238/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1274231/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1371608/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1240876/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1238175/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1462169/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1466801/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1231709/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1495422/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1498157/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1520185/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1551367/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1612115/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1183144/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1674780/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1702616/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1712350/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1754996/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1773118/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1776373/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1787728/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:109427/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATCg > 1:1877593/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGCCTTTTTCACGGATGATCTCGCCTCGTTCGATCAACGCTTTATCg > 1:763721/1‑66 (MQ=255)aagaaCTGGCTGCGGTTTGTGACTTTTTCACGGATGATCTCGGCTCGTTCGATCAACGCTTTATc > 1:1121590/1‑65 (MQ=255) gCGGTTAGTGCCCTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTAATCAGCGTc < 1:1891844/67‑1 (MQ=255) gCGGTTAGTGCCCTTTTCACGGATGATCTCGGCGCGGTCGATTAACGCTTTATCGTTAATCAGCGTc < 1:1587656/67‑1 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:841968/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:111101/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:966229/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:674478/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:1419063/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:568160/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:513679/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:1628811/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:237485/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:1716898/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:2084759/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:1814145/1‑46 (MQ=255) cTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTa > 1:1959411/1‑46 (MQ=255) cTTTTAACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGtt > 1:1430741/1‑45 (MQ=255) | AAGAACTGGCTGCGGTTAGTGCCCTTTTCACGGATGATCTCGGCTCGTTCGATTAACGCTTTATCGTTAATCAGCGTC > minE/2422818‑2422895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |