| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,428,905 | G→A | 100% | S226S (AGC→AGT) | wzzE ← | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,428,905 | 0 | G | A | 92.6% | 154.8 / 3.0 | 54 | S226S (AGC→AGT) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (50/0); total (52/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGCTGTTCATCCGGCGGTCGTAGATGGCTTTCGC > minE/2428844‑2428937 | gTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGCTGtt < 1:1524838/67‑1 (MQ=255)gTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGCTGtt < 1:857742/67‑1 (MQ=255) tCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGc > 1:1707355/1‑58 (MQ=255) tCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGc > 1:458237/1‑58 (MQ=255) ttaGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:836798/3‑49 (MQ=255) tCAGCGCCTGTTCGATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:830144/1‑49 (MQ=21) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCtt > 1:963267/1‑45 (MQ=25) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:573385/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:593731/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:586803/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:575786/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:779229/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:460233/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:439271/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:438602/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:368013/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:350977/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:596457/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:637927/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:663229/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:293582/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:898080/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:903605/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:9151/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:921796/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:926141/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:94656/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:997736/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:175129/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1221560/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1228734/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1282614/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1295227/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:129761/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1371505/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1436996/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1513468/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1531006/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1577352/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1610128/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:174076/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:348786/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1780707/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1918999/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1938195/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1959761/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:2000290/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:2092351/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:2129897/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:2213465/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:2311159/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:241721/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:281694/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc > 1:1168454/1‑49 (MQ=37) | GTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATTTTCAGCGCCTGCTCAATGCTGTTCATCCGGCGGTCGTAGATGGCTTTCGC > minE/2428844‑2428937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |