Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,428,920	G→A	100%	D221D (GAC→GAT)	wzzE ←	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,428,920	0	G	A	100.0%	160.3 / NA	50	D221D (GAC→GAT)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/0);  total (50/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGCGCCTGCTCAATGCTGTTCATCCGGCGGTCGTAGATGGCTTTCGC  >  minE/2428889‑2428937                                |                  ttaGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:836798/3‑49 (MQ=255) tCAGCGCCTGTTCGATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:830144/1‑49 (MQ=21) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCtt      >  1:963267/1‑45 (MQ=25) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:663229/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:348786/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:350977/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:368013/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:438602/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:439271/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:460233/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:573385/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:575786/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:586803/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:593731/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:596457/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:637927/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:293582/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:779229/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:898080/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:903605/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:9151/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:921796/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:926141/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:94656/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:997736/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:175129/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1221560/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1228734/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1282614/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1295227/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:129761/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1371505/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1436996/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1513468/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1531006/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1577352/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1610128/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:174076/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1168454/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1780707/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1918999/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1938195/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:1959761/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:2000290/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:2092351/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:2129897/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:2213465/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:2311159/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:241721/1‑49 (MQ=37) tCAGCGCCTGTTCAATACTGTTCATCCGGCGATCGTAGATAGCTTTCGc  >  1:281694/1‑49 (MQ=37)                                |                  TCAGCGCCTGCTCAATGCTGTTCATCCGGCGGTCGTAGATGGCTTTCGC  >  minE/2428889‑2428937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑