| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,526,291 | G→A | 100% | P195P (CCG→CCA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,526,291 | 0 | G | A | 88.0% | 151.0 / 10.8 | 58 | P195P (CCG→CCA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base A (0/51); total (7/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGG > minE/2526240‑2526316 | gATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt > 1:1027648/1‑67 (MQ=255) atcaCCCTGAAAGAGCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:2038212/53‑1 (MQ=37) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGTCGGa < 1:297786/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:591171/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:210939/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:2206461/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:22771/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:282018/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:292593/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:329176/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:361393/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:392499/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:433689/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:452836/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:2070249/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:616912/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:63315/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:682887/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:726999/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:734449/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:747705/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:757932/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:814511/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:849184/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:889038/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:906916/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:922041/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:990229/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1908466/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1056588/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1057059/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1275945/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1329866/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1342225/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1479055/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1487980/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1509377/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1558143/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1568480/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1721194/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1903155/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1863778/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1852383/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1845918/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1840401/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1834703/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1753400/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1645195/53‑1 (MQ=255) atcaCCCTGAAAGACCTGATTGCCTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1072568/53‑1 (MQ=38) atcaCCCCGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:300237/53‑1 (MQ=255) tcaCCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:65452/52‑1 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:1483198/1‑65 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:1402430/1‑65 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:59769/1‑65 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:155638/1‑65 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:1106882/1‑65 (MQ=255) caCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGcggcgg > 1:1922219/1‑65 (MQ=255) aGACCTGATTGCTTACCTGGAAGAGAAGCCAGAAATGGCGGa < 1:1264489/42‑1 (MQ=25) | GATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGG > minE/2526240‑2526316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |