Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,553,311	G→T	100%	L22I (CTT→ATT)	yibQ ←	predicted polysaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,553,311	0	G	T	100.0%	132.6 / NA	41	L22I (CTT→ATT)	yibQ	predicted polysaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/41);  total (0/41)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACGATGGCAAGTTTGCCAGCAAGTACGGGGGAGGAGAGCGC  >  minE/2553287‑2553329                         |                   tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGTgcgc  <  1:555/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:470260/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1018954/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2128926/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2208100/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:223738/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:240383/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:320633/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:348266/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:349216/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:353629/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2116762/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:61734/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:67263/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:682415/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:759999/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:803418/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:836270/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:85261/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:871655/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1446409/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1045130/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1093898/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1189699/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1219841/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1242343/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1297117/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1394654/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:142965/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2088526/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1491257/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1515457/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1526133/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1570612/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1712692/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1790454/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:1953261/43‑1 (MQ=255) tGACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2065626/43‑1 (MQ=255)  gACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:823972/42‑1 (MQ=255)  gACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:848759/42‑1 (MQ=255)  gACGATGGCAAGTTTGCCAGCAATTACGGGGGAGGAGAgcgc  <  1:2001680/42‑1 (MQ=255)                         |                   TGACGATGGCAAGTTTGCCAGCAAGTACGGGGGAGGAGAGCGC  >  minE/2553287‑2553329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑