| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,642,494 | G→C | 100% | A42A (GCG→GCC) | gntR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,642,494 | 0 | G | C | 88.3% | 180.6 / 12.7 | 60 | A42A (GCG→GCC) | gntR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base C (53/0); total (60/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTTGATGAACTGGGCTATATTCCCAATCGTGCGC > minE/2642436‑2642531 | gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:2137104/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:1141157/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:1175012/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:2138995/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:1556070/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:1754032/1‑66 (MQ=255)gCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTtg > 1:1372807/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTtgatg > 1:109131/1‑39 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTtgatg > 1:626945/1‑39 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTg > 1:422852/1‑44 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATcgcgcg > 1:753494/1‑65 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATcgcgcg > 1:1483845/1‑65 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:37888/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2113466/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:321572/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2292770/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2278742/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2271635/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2183661/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2180230/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2168033/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:911485/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:379771/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:382852/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:608509/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:638020/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:660442/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:685742/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:731930/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:767879/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:818019/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:850108/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:866515/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1079665/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1558086/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1099934/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1127695/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1197746/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1236378/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1246600/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:132991/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1354184/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1355112/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1369818/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1416822/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1446362/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1536359/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1974843/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1589541/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1636076/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1656006/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1667689/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1680762/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1739354/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1778576/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:186106/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1862403/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1863458/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:18802/1‑66 (MQ=255) ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:190089/1‑66 (MQ=255) | GCCGTTTTTTACGCAACCCGGAGCAGGTTTCCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTTGATGAACTGGGCTATATTCCCAATCGTGCGC > minE/2642436‑2642531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |