Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,707,255 C→T 100% A183A (GCC→GCT)  gph → phosphoglycolate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,707,2550CT100.0% 249.0 / NA 71A183A (GCC→GCT) gphphosphoglycolate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (71/0);  total (71/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCCCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGT  >  minE/2707252‑2707318
   |                                                               
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGata                         >  1:687107/1‑44 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTTAGGCGGCAAAAGCGGCAGGt  >  1:225885/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCa     >  1:430263/1‑64 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCa     >  1:963254/1‑64 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAgg   >  1:993421/1‑66 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAgg   >  1:490890/1‑66 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAgg   >  1:1819114/1‑66 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAgg   >  1:583987/1‑66 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAgg   >  1:707778/1‑66 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAg    >  1:1462351/1‑65 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAg    >  1:1213633/1‑65 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2311223/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2301737/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:233194/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:234158/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2175632/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:28904/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:327029/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:355003/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2126930/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:379893/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:405092/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2175388/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:420444/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:451701/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:499349/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:504249/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:524778/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:539215/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:571143/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:6010/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:685937/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:710917/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:765502/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:811295/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:825944/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:859103/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1329861/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1574096/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1568588/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1559437/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1555419/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1497883/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:148603/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1420966/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1330888/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1627618/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:131345/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1304672/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1281988/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1267733/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1248509/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:120760/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1206136/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1899933/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2160673/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:108531/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2090977/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2070246/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2061200/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2007442/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1922769/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1919289/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:2169206/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:188217/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1801865/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1798173/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1761962/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1758385/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1643625/1‑67 (MQ=255)
tGCTCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGt  >  1:1633203/1‑67 (MQ=255)
   |                                                               
TGCCCCACAACAGATGCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGT  >  minE/2707252‑2707318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑