| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,720,012 | A→G | 100% | D523D (GAT→GAC) | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,720,012 | 0 | A | G | 83.8% | 173.8 / 23.8 | 74 | D523D (GAT→GAC) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/12); new base G (0/62); total (0/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGCTCT > minE/2719956‑2720043 | ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:566748/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1816831/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1912279/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1958333/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1976395/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2009279/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2115201/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2139770/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2154356/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2164180/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2252245/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:267543/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:360122/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:394319/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:473349/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1040532/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:567967/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:578726/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:621371/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:651722/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:652535/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:670734/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:757653/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:766855/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:789885/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:844290/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:863968/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:926236/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:961977/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1549707/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1168975/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1235397/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1246196/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1255263/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1301331/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:136442/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1385500/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:139428/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1796293/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1138822/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1477116/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1495950/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1554530/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1011260/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1579717/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1053246/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:174765/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1624217/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1712337/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1624952/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:109169/67‑1 (MQ=255)ggTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGGCATGGGCGt < 1:162189/67‑1 (MQ=255) ggtGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:697021/64‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2018769/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:648183/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2084172/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1453261/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:2104380/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1602980/66‑1 (MQ=255) gTTGCGGGTCCTCGCTCAGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:296041/66‑1 (MQ=255) tcaGAATCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:804504/49‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:923624/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:539380/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:1160864/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:856981/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:769927/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:736893/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:669108/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:528430/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:254192/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:156829/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:1728348/67‑1 (MQ=255) aTCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGctct < 1:1801621/67‑1 (MQ=255) tCAAGCGAATCGCGTCCTGATAGGCTTCTAAAGCGTCATGGGCGt < 1:1789597/45‑1 (MQ=255) | GGTTGCGGATCCTCGCTAAGAATCAAGCGAATCGCTTCCTGATAGGCTTCTAAAGCATCATGGGCGTTTTTACGCAATAACCCGCTCT > minE/2719956‑2720043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |