Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,748,209	G→A	100%	L94L (CTG→CTA)	secY →	preprotein translocase membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,748,209	0	G	A	97.6%	136.8 / ‑4.1	42	L94L (CTG→CTA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (0/41);  total (0/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCTTCTATCTTTGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTGCTGACG  >  minE/2748149‑2748215                                                             |       tGCTTCTATCTTTGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTGCTGACg  <  1:1199545/67‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:452479/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2009216/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2023999/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:209505/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:211496/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2175258/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2234842/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2257908/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:2316267/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:259318/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:287454/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1938799/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:511325/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:534299/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:555903/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:571356/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:60258/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:65854/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:726259/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:987419/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1429888/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1025768/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1026423/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1028307/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1153768/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1223368/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:12308/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1294558/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1407725/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1003714/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1441597/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:155966/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1612177/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1645251/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1687517/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1720322/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:186367/53‑1 (MQ=255)             tGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1876403/53‑1 (MQ=255)              gCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1118030/52‑1 (MQ=255)                       aTCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:1500907/43‑1 (MQ=255)                         cATGCCGTATATTTCGGCGTCGATCATTATCCAGCTACTGa    <  1:841995/41‑1 (MQ=255)                                                             |       TGCTTCTATCTTTGCTCTGGGGATCATGCCGTATATTTCGGCGTCGATCATTATCCAGCTGCTGACG  >  minE/2748149‑2748215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑