Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,919,561	A→G	100%	G45G (GGT→GGC)	argI ←	ornithine carbamoyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,919,561	0	A	G	100.0%	166.4 / NA	49	G45G (GGT→GGC)	argI	ornithine carbamoyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/0);  total (49/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTT  >  minE/2919544‑2919584                  |                        aTGAGCGCGTTGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:360664/1‑41 (MQ=37) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:116548/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:1212901/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:66640/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:613931/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:53665/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:1557901/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGcttcttctt   >  1:527450/1‑40 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2210391/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2117717/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2232217/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2256123/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2258778/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2296235/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:284280/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:388616/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:454496/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:509899/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:53164/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:565806/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:607752/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:850098/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:962167/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:992119/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:992155/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1822033/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1037778/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1128819/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1146203/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1173056/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1240503/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1344682/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:139021/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1473353/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:152758/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1722757/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1805614/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1827288/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:187952/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:19255/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1967904/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:202405/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2025158/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2080597/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:2111080/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTCTTCttt  >  1:1013570/1‑41 (MQ=38) aTGAGCGCGATGTTTTTGCCAGTGAGTCTGGCTTATTCttt  >  1:2132281/1‑41 (MQ=37) aTGAGCGCGATGTTTTTGCCAGTCAGTCTGGCTTCTTCttt  >  1:380389/1‑41 (MQ=37) aTGAGCGCGATGTTTTTGCCAGTCAGTCTGGCTTCTTCttt  >  1:377754/1‑41 (MQ=37)                  |                        ATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTTT  >  minE/2919544‑2919584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑