Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 317,881 G→T 100% G959G (GGG→GGT kefA → fused mechanosensitive channel proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE317,8810GT86.0% 219.0 / 38.1 100G959G (GGG→GGTkefAfused mechanosensitive channel proteins
Reads supporting (aligned to +/- strand):  ref base G (14/0);  new base T (0/86);  total (14/86)
Fisher's exact test for biased strand distribution p-value = 2.26e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTCTATTCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAT  >  minE/317822‑317923
                                                           |                                          
aTTCTGTTCGAGCGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:100012/67‑3 (MQ=25)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTATGgtca                                     <  1:149202/67‑3 (MQ=25)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2234422/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:381081/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:335055/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:302004/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:299984/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:266045/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:256377/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:244645/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:237646/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:236782/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:383820/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2188511/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2182180/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2121340/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2098376/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2023520/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2017252/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:200621/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2001783/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1966254/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:743118/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:993426/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:986311/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:98050/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:970464/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:96694/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:916867/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:881551/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:823833/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:814194/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:744301/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2053272/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:735310/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:727292/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:705011/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:643262/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:630799/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:628262/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:586955/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:538106/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:53541/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:476700/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1360773/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1478481/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1466071/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1461875/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1413445/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1042815/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:107323/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1391160/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:110144/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1458761/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1107510/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1351808/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1120794/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1327914/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1309518/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1247642/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1227902/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1133646/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1710798/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1893153/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:186368/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1852991/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1831346/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1784670/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1935754/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:177529/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1757068/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1745807/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1607800/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1649253/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1634371/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1620916/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1617990/67‑3 (MQ=37)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACCgtca                                     <  1:297991/67‑3 (MQ=25)
aTTCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTAAGgtca                                     <  1:1409616/67‑3 (MQ=25)
aTTCTGTTCGAACGTCCGGGGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1260569/67‑3 (MQ=255)
aTTCTATTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:2024685/67‑3 (MQ=37)
 ttCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:95703/66‑3 (MQ=37)
 ttCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:549985/66‑3 (MQ=37)
 ttCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1036705/66‑3 (MQ=37)
 ttCTGTTCGAACGTCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1042160/66‑3 (MQ=37)
          aaCGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCg                           >  1:1448184/1‑67 (MQ=255)
              tCCGGTGCGCATAGGCGACACGGTAACCATTGGTAGCTTCTCGGGTACGgtca                                     <  1:1037586/53‑3 (MQ=25)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:915697/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:1023503/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:639879/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:630380/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:1369954/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:562101/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:1407980/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:466985/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:425113/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:398107/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:35460/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:2239528/1‑67 (MQ=255)
                                   ggTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAt  >  1:2059128/1‑67 (MQ=255)
                                                           |                                          
ATTCTATTCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGAT  >  minE/317822‑317923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: