| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 400,660 | A→G | 100% | F292F (TTT→TTC) | dacA ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 400,660 | 0 | A | G | 81.8% | 93.2 / 14.8 | 44 | F292F (TTT→TTC) | dacA | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base G (36/0); total (44/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAACCAAACCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCA > minE/400602‑400705 | caaacgaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1367519/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGac > 1:2186084/1‑63 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:616286/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1092106/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1967676/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:2066466/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:2120686/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:2246763/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:2296182/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:265693/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:526956/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1857671/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:629246/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:639885/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:709150/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:734652/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:741178/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:863740/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:878347/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:934797/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1098600/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1262691/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1201852/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1520686/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1525658/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1565659/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1569957/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1573333/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1182053/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1175858/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1629763/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:169961/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1705708/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1706003/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCGAAGAAAc > 1:1797674/1‑66 (MQ=255)caaaccaaacCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGATAACGGTTTCGAAGAAAc > 1:1806191/1‑66 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTTCTTTCGGCTTCa > 1:572118/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:1186499/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:516669/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:1284985/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:2262741/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:2260064/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:159890/1‑67 (MQ=255) tttCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCa > 1:1843315/1‑67 (MQ=255) | CAAACCAAACCGGTTCAGAGGCGAACTCTTTACCTACTTTCAGTGGGTTAACGGTTTCAAAGAAACGGAAGCCCCAGGTTAGCAGTTTTTTACTTTCGGCTTCA > minE/400602‑400705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |