| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 498,441 | C→T | 38.7% | G24S (GGT→AGT) | galE ← | UDP‑galactose‑4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 498,441 | 0 | C | T | 38.7% | 146.8 / 212.7 | 197 | G24S (GGT→AGT) | galE | UDP‑galactose‑4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (44/76); new base T (38/38); total (82/115) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01 | |||||||||||
Only 100 of 197 total aligned reads displayed.
ACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAC > minE/498383‑498504 | aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:454393/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:2028014/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:2015150/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:2201245/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:1722295/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:327547/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:1626288/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:430833/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:1500040/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:1367916/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:696959/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCa < 1:819626/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:3565/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1002153/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:2134014/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1944285/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:219011/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1758288/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1053733/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:897749/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1702966/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:298418/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1225637/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1168850/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:767670/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:46481/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:502886/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:518730/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1385006/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:56862/67‑1 (MQ=255)aCAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGACGACATCATGACCGTTTTGCa < 1:2104644/67‑1 (MQ=255) cAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:1735878/66‑1 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:645046/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:37762/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:953021/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:576915/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:575351/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:2144575/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:2149919/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:291342/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:733067/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:100955/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:427005/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1604211/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:169245/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1213522/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:165466/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1361967/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1427546/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1045736/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1485114/1‑67 (MQ=255) cTGCGCTTACTGTTACAGAGGTTATCAAGAATGAAGACATCATGACTGTTTTGCAGTAATTGcacac > 1:1478734/1‑67 (MQ=255) cgcTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCa < 1:376249/52‑1 (MQ=255) agagGTAATCAAGAATGATGACATCATGACCGTTTTGCa < 1:2148460/39‑1 (MQ=255) gagGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACTGGTATGACTTCCAAt < 1:293411/66‑1 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:836729/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:2238417/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:2269010/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:404399/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:277046/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTaa > 1:960351/1‑67 (MQ=255) gTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTa > 1:508979/1‑66 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCGCACAGGTATGACTTCCAATGTAACCGCt < 1:773428/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1218204/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:684718/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1187563/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:614060/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1346352/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:776841/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1142613/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:1835103/57‑1 (MQ=255) gaCATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCt < 1:2052072/57‑1 (MQ=255) atcatGACGGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:2167470/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:914090/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:199177/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:1787734/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:1773187/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:2295311/67‑1 (MQ=255) atcatGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAAcc < 1:157005/67‑1 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCg > 1:1705766/1‑56 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGaa > 1:284809/1‑66 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:721699/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:970601/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1977827/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:955128/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1090504/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:2213260/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:248072/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1687824/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:33353/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1308089/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:617117/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:361410/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:611062/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1317366/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:1613319/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:159321/1‑67 (MQ=255) gACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:534571/1‑67 (MQ=255) gACCGTTTTGCAGTAACTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAc > 1:175792/1‑67 (MQ=255) gACCGTATTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCg > 1:596795/1‑56 (MQ=255) | ACAGGCAGTACGCTGCGCTTACTGTTACAGAGGTTATCAAGAATGATGACATCATGACCGTTTTGCAGTAATTGCACACAGGTATGACTTCCAATGTAACCGCTACCACCGGTAACCAGAAC > minE/498383‑498504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |