| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 531,823 | G→A | 100% | I247I (ATC→ATT) | ybhR ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 531,823 | 0 | G | A | 79.6% | 98.5 / 20.4 | 49 | I247I (ATC→ATT) | ybhR | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/10); new base A (39/0); total (39/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGC > minE/531787‑531864 | cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:875240/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:207359/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:691709/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:2190269/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:608723/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:1839356/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:508378/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:2247640/67‑1 (MQ=255)cGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:1611486/67‑1 (MQ=255) gTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGc < 1:172740/66‑1 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:289221/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2128217/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2164518/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2200237/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2220985/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2227308/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2280098/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:287337/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1936637/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:331561/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:489504/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:54682/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:586576/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:637032/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:691318/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:792702/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:877170/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1480232/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1010878/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1023113/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1056706/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1091361/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1156542/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:122105/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1334185/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1363828/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1404023/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:2080651/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1491069/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1605761/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1695385/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1699799/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1744031/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1795233/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:183164/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1885542/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:1904525/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGc > 1:10048/1‑50 (MQ=255) gCGAAGGGAATTTGATACGCCCAGATACAAATCGCCAGCACAAtggt > 1:1450790/1‑47 (MQ=39) | CGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGC > minE/531787‑531864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |