Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 64,708 G→A 100% S299S (TCC→TCT)  leuC ← 3‑isopropylmalate isomerase subunit, dehydratase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE64,7080GA100.0% 259.1 / NA 78S299S (TCC→TCT) leuC3‑isopropylmalate isomerase subunit, dehydratase component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (78/0);  total (78/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAA  >  minE/64646‑64711
                                                              |   
tCTGCGGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:236063/1‑66 (MQ=255)
tCTGCCGCCGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1939956/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2288218/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:383553/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:350606/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:345548/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:34475/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:25207/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2312085/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2304977/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2298411/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:384276/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2180444/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2178637/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2135340/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2081701/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2055222/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2034940/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:2006104/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1919096/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:622501/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:961773/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:943677/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:926957/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:894377/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:768831/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:763845/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:749195/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:738275/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:701605/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1916429/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:576056/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:565647/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:542290/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:510315/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:491908/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:460870/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:404199/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:39935/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1141295/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1322832/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1292841/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1270847/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:126608/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1186447/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1178197/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:116874/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1161477/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1147121/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1389134/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1136723/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1118203/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1110349/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1086454/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1083155/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:105400/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1046147/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1016635/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1011182/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1665450/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1911091/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1891623/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1885371/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1773193/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1767533/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:175292/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1740152/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1705690/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1695411/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1006836/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1658274/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1649573/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1558398/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1544885/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1526178/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1518956/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1502629/1‑66 (MQ=255)
tCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACAGaa  >  1:1442100/1‑66 (MQ=255)
                                                              |   
TCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAA  >  minE/64646‑64711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑