Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 564,247 A→G 100% Y218Y (TAT→TAC)  ybjL ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE564,2470AG96.1% 157.5 / ‑2.8 50Y218Y (TAT→TAC) ybjLpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (48/0);  total (50/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGC  >  minE/564195‑564285
                                                    |                                      
gTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCAc                          >  1:1597400/1‑67 (MQ=255)
gTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCAc                          >  1:803906/1‑67 (MQ=255)
                        tCCAGGGCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:620059/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGCCGCGGTAGGCGCgg                               >  1:2282782/1‑38 (MQ=25)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGcgcg                                >  1:24858/1‑37 (MQ=38)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGtaaataaa               >  1:1951558/1‑54 (MQ=39)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTgg   >  1:138288/1‑66 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTgg   >  1:610360/1‑66 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTgg   >  1:1649912/1‑66 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTgg   >  1:141518/1‑66 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTgg   >  1:228822/1‑66 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:360378/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:2177796/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:431702/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:546844/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:2261524/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:2258802/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1966879/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:640767/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:665851/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:692906/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:697982/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:769899/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:775623/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:840295/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:84175/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:860203/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:948966/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1451503/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1068008/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1132147/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1221140/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1280811/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1302815/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1303622/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1357429/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1371183/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1389122/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1423266/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:213092/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:152286/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1671111/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1677472/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1704934/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1729585/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:180896/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1882317/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1888951/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:1044863/1‑67 (MQ=255)
                        tCCAGGCCACCAGCTCCGGGCCGACGCGGTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGc  >  1:2110409/1‑67 (MQ=255)
                                                    |                                      
GTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGC  >  minE/564195‑564285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑