| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 565,810 | A→G | 100% | S9P (TCG→CCG) | grxA ← | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 565,810 | 0 | A | G | 95.1% | 126.0 / ‑1.6 | 41 | S9P (TCG→CCG) | grxA | glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (0/39); total (0/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAA > minE/565767‑565865 | ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:648155/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2281562/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2303888/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:254035/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:358157/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:429322/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:442886/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:459431/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:498195/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:583089/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2199452/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:821367/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:86375/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:902265/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:902450/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:925906/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:934628/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:935936/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:956450/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:987511/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1785740/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1223355/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1323517/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1377593/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1388809/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1531975/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1569270/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1572515/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1668783/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1748898/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:118219/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1791887/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1893842/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:1983547/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2036645/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2115451/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2131606/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2137886/52‑1 (MQ=255)ttCTCGGCCAGATCTTTGGCACGCACACAGTAAGGGCAACCCGGACGACCaa < 1:2139629/52‑1 (MQ=255) aGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTaa < 1:709305/67‑1 (MQ=255) aGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTaa < 1:1976671/67‑1 (MQ=255) | TTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAA > minE/565767‑565865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |