| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 599,873 | G→T | 100% | G739V (GGC→GTC) | dmsA → | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 599,873 | 0 | G | T | 100.0% | 97.7 / NA | 29 | G739V (GGC→GTC) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (29/0); total (29/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATCCACAACGGCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGT > minE/599861‑599927 | gTATCCACACCGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGaa > 1:481845/1‑64 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGaa > 1:978442/1‑64 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:258068/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:825345/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:736606/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:701742/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:69321/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:692459/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:585982/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:559223/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:552375/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:503875/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:457713/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:436307/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:301752/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1071670/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:232292/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:2236595/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:215659/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1967304/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1965615/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1933194/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1830899/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1663459/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1644064/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1523874/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1212634/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1142826/1‑67 (MQ=255)gTATCCACAACGTCGATAAAGTCAGGATCTGTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGt > 1:1963812/1‑67 (MQ=255) | GTATCCACAACGGCGATAAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGT > minE/599861‑599927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |