| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 634,389 | T→C | 100% | T455T (ACT→ACC) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 634,389 | 0 | T | C | 93.6% | 187.3 / 2.6 | 62 | T455T (ACT→ACC) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base C (0/58); total (4/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.79e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGT > minE/634367‑634447 | aGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATgg > 1:99699/1‑60 (MQ=255)aGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAAc > 1:2298926/1‑67 (MQ=255)aGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAAc > 1:1504419/1‑67 (MQ=255)aGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAAc > 1:134665/1‑67 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCAcagtcagt < 1:354069/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:545273/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2237819/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:52005/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:451403/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:346212/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:324770/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:294732/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:249311/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2301524/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2258572/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2256487/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2251175/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2159707/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:615020/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:641407/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:642228/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:655770/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:772658/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:778345/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:831453/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:8587/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:861725/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:869477/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:958540/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:962290/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:974367/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:980037/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1682301/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1051309/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1057402/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:10696/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1199106/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1233317/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1262836/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1338706/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1371142/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1378537/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1403448/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1534593/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1597586/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1639675/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2191207/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1740729/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:175286/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1756422/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1766586/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1852808/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1856176/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1862950/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1868049/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1960053/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1966590/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1985907/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:2117670/65‑1 (MQ=255) aGCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1013105/65‑1 (MQ=255) gCGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:1413227/64‑1 (MQ=255) cGACCGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGt < 1:193881/63‑1 (MQ=255) | AGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGT > minE/634367‑634447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |