| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 755,969 | C→G | 100% | V32V (GTC→GTG) | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 755,969 | 0 | C | G | 84.1% | 108.0 / 15.3 | 44 | V32V (GTC→GTG) | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (6/1); new base G (0/37); total (6/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.92e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTA > minE/755934‑756015 | cAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAat < 1:2279322/50‑1 (MQ=255) aaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:1038025/1‑55 (MQ=255) aaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:2228648/1‑55 (MQ=255) aaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:1325317/1‑55 (MQ=255) aaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:883225/1‑55 (MQ=255) aaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:466741/1‑55 (MQ=255) aaCAGATGTGCTGCACAATGTCAGTTTCAGCGTCGGc > 1:444745/1‑37 (MQ=255) tCAGCGTGGTCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1698844/54‑1 (MQ=38) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2206781/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2105006/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2212825/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:346036/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:371476/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:446080/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:515292/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:586749/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:59832/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:676751/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:820387/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:823148/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:900776/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:960248/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:983947/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2131158/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1171793/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1201039/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:124844/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1398850/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1419188/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1498000/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1565690/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1622591/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:162896/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:170723/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1713030/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1812553/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1839470/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1924209/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1943004/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2016836/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2101819/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1037590/54‑1 (MQ=39) tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGGCGGCAGCTCTGGTTCCGGTa < 1:995987/54‑1 (MQ=255) cAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1692050/53‑1 (MQ=39) | CAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTA > minE/755934‑756015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |