| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 755,999 | T→C | 100% | G42G (GGT→GGC) | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 755,999 | 0 | T | C | 79.2% | 91.6 / 20.5 | 48 | G42G (GGT→GGC) | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/10); new base C (0/38); total (0/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGC > minE/755962‑756045 | tCAGCGTGGTCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1698844/54‑1 (MQ=38)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:446080/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2016836/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2101819/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2105006/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2131158/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2206781/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:2212825/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:346036/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:371476/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1037590/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:515292/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:586749/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:59832/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:676751/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:820387/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:823148/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:900776/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:960248/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:983947/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1924209/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1839470/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1812553/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1713030/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:170723/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1943004/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1171793/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1201039/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:124844/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:162896/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1398850/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1622591/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1565690/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1419188/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1498000/54‑1 (MQ=39)tCAGCGTGGGCGAAGGTGAAATGATGGCGATCGGCGGCAGCTCTGGTTCCGGTa < 1:995987/54‑1 (MQ=255) cAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1692050/53‑1 (MQ=39) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:1514845/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:627686/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:542215/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:68972/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:737891/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:408010/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:364093/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:23162/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:966392/67‑1 (MQ=255) gAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggcgggc < 1:2262033/67‑1 (MQ=255) aaaTGATGGCGATCGTCGGCAGCTCTGGTTCCGGTa < 1:1019296/36‑1 (MQ=255) | TCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGC > minE/755962‑756045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |