Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	770,702	G→A	100%	I180I (ATC→ATT)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	770,702	0	G	A	100.0%	58.4 / NA	18	I180I (ATC→ATT)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/18);  total (0/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACC  >  minE/770678‑770728                         |                           ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGTCTGGCTGAcc  <  1:770930/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:222765/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:887593/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:804467/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:625089/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:465355/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:33321/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:2267843/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:2255078/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1114850/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:216984/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:2046518/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:196998/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1883593/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1791110/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1708766/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1691795/51‑1 (MQ=255) ggCGACGTTAGCCATCTCTTTACCAATGGTTGACGGCGTGGCTGGCTGAcc  <  1:1439549/51‑1 (MQ=255)                         |                           GGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACC  >  minE/770678‑770728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑