Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	799,282	T→C	100%	E103E (GAA→GAG)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	799,282	0	T	C	100.0%	134.2 / NA	40	E103E (GAA→GAG)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/0);  total (40/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCCTCTGGCGGAACATATTTCTCGCCTTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATGG  >  minE/799255‑799318                            |                                     ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAAc         >  1:176680/1‑57 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:559201/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:2055228/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:2184299/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:2299299/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:2310353/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:270630/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:28834/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:352339/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:463072/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:2040937/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:573708/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:771921/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:806101/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:81295/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:824853/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:851251/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:944274/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:975668/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:976183/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1456600/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1093512/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1128867/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1179652/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1206517/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1280918/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1295213/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1358014/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1377620/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1490842/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1492803/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1530430/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1652167/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1794232/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1800797/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1810211/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:200209/1‑64 (MQ=255) ccccTCTGGCGGAACATATTTCTAGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1405368/1‑64 (MQ=255) ccccTCTGGCGGAACATATGTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:1056702/1‑64 (MQ=255) caccTCTGGCGGAACATATTTCTCGCCCTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATgg  >  1:383624/3‑64 (MQ=255)                            |                                     CCCCTCTGGCGGAACATATTTCTCGCCTTCTTTCGGCAGGGTGAAATTGACGTTAACGAAATGG  >  minE/799255‑799318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑