Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	807,098	T→C	100%	T357A (ACC→GCC)	ycgV ←	predicted adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	807,098	0	T	C	89.7%	104.1 / 3.7	40	T357A (ACC→GCC)	ycgV	predicted adhesin
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base C (35/0);  total (40/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGC  >  minE/807080‑807154                   |                                                         gATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCAcc             >  1:2166175/1‑64 (MQ=255) gATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCAcc             >  1:357152/1‑64 (MQ=255) gATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCAcc             >  1:1690144/1‑64 (MQ=255)         gTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:388375/1‑67 (MQ=255)         gTAATATCGGGATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1704802/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAgg                 >  1:252439/1‑52 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAg        >  1:536028/1‑61 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:345816/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:2042294/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:2104549/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:222708/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:2231572/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:335357/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:2007902/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:365932/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:632531/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:732402/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:763042/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:884582/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:977568/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1521286/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1176291/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1203711/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1348526/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1383615/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1415189/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1448870/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1467275/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1515080/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1111169/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1599168/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1713378/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1731583/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1769741/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1801965/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1865623/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1908660/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:1944823/1‑67 (MQ=255)         gTAATATCGGCATTTTGCATGTTGACAACAACCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:675130/1‑67 (MQ=255)         gTAAAATCGGCATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgc  >  1:299361/1‑67 (MQ=255)                   |                                                         GATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGC  >  minE/807080‑807154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑