| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 828,767 | T→A | 100% | R177R (CGA→CGT) | narX ← | sensory histidine kinase in two‑component regulatory system with NarL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 828,767 | 0 | T | A | 100.0% | 25.8 / NA | 11 | R177R (CGA→CGT) | narX | sensory histidine kinase in two‑component regulatory system with NarL |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/0); total (11/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGTCGCGCCCGCAACCAGATAAT > minE/828721‑828787 | tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:1145078/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:1402134/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:1539648/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:1884586/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:2122327/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:213937/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:2266030/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:370572/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:527404/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:80105/1‑67 (MQ=255)tgacGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGACGCGCCCGCAACCAGataat > 1:814030/1‑67 (MQ=255) | TGACGGCACTCGCCATTGCCAGCAGTTGCCGCCACGGTTGTAGCAGTCGCGCCCGCAACCAGATAAT > minE/828721‑828787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |