| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 891,136 | C→T | 100% | L151L (TTG→TTA) | sapF ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 891,136 | 0 | C | T | 84.6% | 25.6 / ‑0.6 | 13 | L151L (TTG→TTA) | sapF | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (11/0); total (11/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCG > minE/891085‑891191 | gcgcAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATa < 1:1933076/66‑1 (MQ=255)gcgcAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATa < 1:2123768/66‑1 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1173967/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1433857/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1467292/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1559859/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1740025/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:1766038/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:2025533/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:2130485/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:732857/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:839315/4‑67 (MQ=255) tgcccGGGTGCTAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCg > 1:914682/4‑67 (MQ=255) | GCGCAATATCAGCGCGCGCGCCAGACCCAGACGCTGTTTTTGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCG > minE/891085‑891191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |