| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,172 | A→G | 100% | N665N (AAT→AAC) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,172 | 0 | A | G | 84.8% | 306.6 / 40.7 | 125 | N665N (AAT→AAC) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (18/1); new base G (36/70); total (54/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.23e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 125 total aligned reads displayed.
CCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCT > minE/138113‑138219 | ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1656700/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1344200/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2047622/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1429059/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:327932/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1502314/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1504186/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2275440/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1628798/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2205021/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:476897/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1669213/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1686330/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1989097/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1704501/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1988581/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1722405/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:466658/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:818785/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2195804/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1088324/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:1142772/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2131139/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2259045/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:908994/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:188318/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:900052/66‑1 (MQ=255)ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:876205/66‑1 (MQ=255) aTACAAAACGACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:794005/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:577963/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1536446/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1967407/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1280402/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1459796/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:2045496/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:299270/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:2150401/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1413243/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1055559/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1040093/1‑67 (MQ=255) aTACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAAcc > 1:1755267/1‑67 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:2278878/54‑1 (MQ=255) ggTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCa < 1:628634/40‑1 (MQ=255) tGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATg < 1:1202060/66‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1976157/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:2026875/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:226832/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1948157/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1028997/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1713034/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:169870/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:495772/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:513056/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:76494/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:769239/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:851138/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1170762/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1100044/49‑1 (MQ=255) gaAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCg < 1:1071460/49‑1 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:282113/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:376497/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:433609/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:952324/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:996211/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:2258413/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1540243/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1031215/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1198293/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1199453/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1272139/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1280932/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1282025/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1325150/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:145425/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1502077/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:2241476/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1733504/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1831587/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:2101561/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:2025690/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1861433/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1771607/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATa > 1:1774326/1‑67 (MQ=255) aaaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCACCATGGTGACTCATa > 1:542634/1‑67 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGACCCTGCCAGCGtt > 1:1598514/1‑49 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGtt > 1:661866/1‑49 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGtt > 1:1515529/1‑49 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGtt > 1:858249/1‑49 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGtt > 1:2311275/1‑49 (MQ=255) aaCTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGtt > 1:1660144/1‑49 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:613069/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:330799/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:1355777/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:1341749/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:870510/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:194284/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:2093843/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:428349/67‑1 (MQ=255) cTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCCGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:505733/67‑1 (MQ=255) tATCGCTGTTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCt < 1:1877648/55‑1 (MQ=255) | CCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCT > minE/138113‑138219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |