Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,036,743	G→A	100%	V712I (GTT→ATT)	rsxC →	fused predicted 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,036,743	0	G	A	100.0%	61.7 / NA	39	V712I (GTT→ATT)	rsxC	fused predicted 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/0);  total (39/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCA  >  minE/1036689‑1036755                                                       |             gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCTAGAAGAACAGATTGATCcgcg    >  1:1498652/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTTATCcgcg    >  1:1918306/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATc        >  1:1747083/1‑61 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1926143/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1675542/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1743379/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1772201/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1824452/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1895463/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1897339/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:166412/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1930292/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1996727/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:2051095/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:24831/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:439723/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:551314/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:612318/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:80230/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:982537/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1452847/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1066198/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1093356/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1154426/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1163349/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1186827/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1305283/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1420661/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1424826/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1002915/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1462216/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1486478/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1491464/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1505382/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1529115/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1545838/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgcg    >  1:1644178/1‑65 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCcgc     >  1:1870734/1‑64 (MQ=11) gcCAAAGCACGTAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCa  >  1:1393799/1‑67 (MQ=11)                                                       |             GCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCA  >  minE/1036689‑1036755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑