BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,063,122	C→A	100%	H18Q (CAC→CAA)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,063,122	0	C	A	100.0%	54.1 / NA	17	H18Q (CAC→CAA)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/17); total (0/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAA > minE/1063102‑1063187

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:940792/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:2283627/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:241314/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:479070/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:505641/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:2130652/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1413409/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1410373/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:627082/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:627405/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1874783/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1955926/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1249869/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:804532/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:840094/67‑1 (MQ=255)

aaaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:10496/67‑1 (MQ=255)

 aaGATGCTCTGGCACCGCAAATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGa                     <  1:1361767/66‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2096/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2105345/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2117009/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2050858/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2165296/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1950741/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2251778/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:2008046/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:401922/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:543335/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:550377/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:610447/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:62193/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:641463/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:718533/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:791110/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:811688/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:842356/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1458848/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1020853/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1076962/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1126414/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:119528/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1283509/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1324195/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:133352/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1345088/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1416708/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1441179/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1452323/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1995382/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1475601/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1477864/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:15158/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1577500/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1587706/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1642582/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:165829/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1668581/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1734227/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1872735/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1015347/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:195906/67‑1 (MQ=255)

                   acaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:198028/67‑1 (MQ=255)

                    caTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:140548/66‑1 (MQ=255)

                    caTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGaa  <  1:1240680/66‑1 (MQ=255)

AAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAA > minE/1063102‑1063187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`