| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,066,096 | C→T | 100% | L188L (CTG→TTG) | purR → | DNA‑binding transcriptional repressor, hypoxanthine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,066,096 | 0 | C | T | 100.0% | 43.9 / NA | 16 | L188L (CTG→TTG) | purR | DNA‑binding transcriptional repressor, hypoxanthine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/16); total (0/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAG > minE/1066074‑1066140 | aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:1186551/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:145833/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:1551847/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:1741622/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:187588/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:1998763/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:2022536/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:2119832/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:314815/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:32826/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:562299/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:851424/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:9201/67‑1 (MQ=255)aaTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:963919/67‑1 (MQ=255)aaTCGGCGGCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:948765/67‑1 (MQ=255) aTCGGCGTCATCCCCGGCCCGTTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg < 1:1597415/66‑1 (MQ=255) | AATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAG > minE/1066074‑1066140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |