| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,077,998 | T→C | 100% | L14L (TTA→TTG) | ydhU ← | predicted cytochrome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,077,998 | 0 | T | C | 80.3% | 155.2 / 32.7 | 76 | L14L (TTA→TTG) | ydhU | predicted cytochrome |
| Reads supporting (aligned to +/- strand): ref base T (10/5); new base C (61/0); total (71/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAG > minE/1077950‑1078020 | cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:398564/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2123999/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2132776/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2211967/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:921070/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2239356/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2245847/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2265271/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2281000/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2306056/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:245573/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:262396/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:282541/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:91818/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:362880/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:395502/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:2020940/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:898791/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:441271/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:451690/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:457020/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:488725/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:89151/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:526474/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:628719/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:639474/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:647632/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:673685/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:674179/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:851472/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:744643/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:791786/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:9724/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1036394/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1121131/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1150103/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1169885/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1188852/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1386950/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:976445/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1475439/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1491533/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1501565/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1511004/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1525767/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1590814/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1954656/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:164602/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:167726/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1696490/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1954058/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1928093/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1730588/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:190471/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1767658/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1768778/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:177620/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:180994/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1815699/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTGTGGAACATAGTTCGCCAACTGGCTCTGAAACTg > 1:40683/1‑66 (MQ=255)cAACCACACCGGCCAAAATTGGGGGGTGAACTCTGGCACATAGTTCGCCAACTGGCTCTGAAACTg > 1:1606520/1‑66 (MQ=255) ccACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTc < 1:1753869/66‑1 (MQ=255) cTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTc < 1:1164816/39‑1 (MQ=255) cTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTc < 1:1555263/39‑1 (MQ=255) cTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTc < 1:1568111/39‑1 (MQ=255) tgtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTc < 1:2188906/38‑1 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCa > 1:995950/1‑38 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:762839/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:724744/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:519963/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:406440/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:3050/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:2232945/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:1605622/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:147538/1‑39 (MQ=255) gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAg > 1:1004368/1‑39 (MQ=255) | CAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAG > minE/1077950‑1078020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |