Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,083,088	A→T	100%	intergenic (‑256/‑301)	ydhZ ← / → pykF	hypothetical protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,083,088	0	A	T	97.8%	155.0 / ‑3.8	46	intergenic (‑256/‑301)	ydhZ/pykF	hypothetical protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (45/0);  total (45/1)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGA  >  minE/1083071‑1083134                  |                                               aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:362784/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1082007/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2026853/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2033064/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2104130/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2139574/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2210057/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2290202/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2315873/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:243433/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:27468/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:348758/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:2012548/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:365827/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:409949/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:412331/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:569868/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:598152/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:650052/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:674358/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:687514/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:913865/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:96603/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1491861/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1097122/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1161582/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1229459/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1281151/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1299096/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1331787/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1405437/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1413875/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1417051/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:146477/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1903542/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1502351/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1520573/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1537794/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1538975/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1577069/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1656183/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1720961/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1753935/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:1829305/1‑46 (MQ=255) aTAACAATTTATTGTCTTACAAGTTGTATATTTTTTGAAACGCtgt                    >  1:188815/1‑46 (MQ=255) aTAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGa  <  1:670780/64‑1 (MQ=255)                  |                                               ATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGA  >  minE/1083071‑1083134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑