| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 199,427 | A→T | 100% | T109T (ACT→ACA) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 199,427 | 0 | A | T | 80.9% | 307.3 / 63.6 | 131 | T109T (ACT→ACA) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/25); new base T (106/0); total (106/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 131 total aligned reads displayed.
CAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCA > minE/199411‑199478 | cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1988565/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:540281/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1665881/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1771322/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:458294/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1482530/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:715012/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:37964/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:772749/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:778119/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:298354/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:127992/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1048737/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:86032/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:2120254/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:933524/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1167497/67‑1 (MQ=255)cAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc < 1:1163360/67‑1 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc > 1:815975/1‑66 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATc > 1:441974/1‑66 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2177034/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2267014/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2201751/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:538096/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:525382/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:523224/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:515909/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:450274/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2204862/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:371031/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:221237/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:667318/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2280674/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2220665/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:764409/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:973239/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:964270/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:954166/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:930594/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:888294/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:84885/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:841279/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:825168/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:798952/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:786054/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:544083/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:717419/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:697194/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:689150/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:680772/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2118096/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:630325/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:619418/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:594910/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:585602/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1288441/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:155415/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1546130/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1520419/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1475557/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1451339/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1409624/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1403835/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1336020/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1331362/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:131832/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1295279/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:156306/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1287198/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:125370/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1247835/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1234211/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1217634/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1207116/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1182864/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:116444/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1079682/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1044481/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1915210/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2149467/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2144369/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1011101/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2109187/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2087378/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2061972/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:2053673/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1982413/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1968876/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1957399/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:216302/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1894025/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1893333/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:186775/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1837987/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:183648/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1764145/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1722317/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1645763/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1639115/1‑67 (MQ=255) aaTCGGATAAACAAATGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCa > 1:1592911/1‑67 (MQ=255) | CAATCGGATAAACAAAAGTGTTGCCTACTGCGACCAGTTTGTAGCCACGATCTTTCAGTTGCTGATCA > minE/199411‑199478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |