| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,105,255 | G→A | 100% | A123A (GCG→GCA) | ydiO → | predicted acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,105,255 | 0 | G | A | 84.4% | 199.4 / 26.0 | 83 | A123A (GCG→GCA) | ydiO | predicted acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (8/5); new base A (0/70); total (8/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAA > minE/1105198‑1105315 | gcTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGaa < 1:1699184/67‑1 (MQ=255)gcTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGaa < 1:1055082/67‑1 (MQ=255)gcTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGaa < 1:863861/67‑1 (MQ=255)gcTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGaa < 1:660877/67‑1 (MQ=255) cTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGaa < 1:1070906/66‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACCACAGTGCCACTACCACTTacac < 1:402949/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACTGTGCCACTACCACTTacac < 1:2302506/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:228796/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:333839/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:315281/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:30566/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:274860/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:262491/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:254073/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:252523/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:398066/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2281140/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2213889/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2210961/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2188863/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:875799/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2142947/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:639907/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:778482/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:736380/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:700160/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:694273/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:684107/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:681855/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:661761/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:657060/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:337042/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:603191/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:579566/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:779844/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:531874/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:518016/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:40098/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2121630/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:356258/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1225514/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:970973/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1398327/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1364300/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1342175/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1305905/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:993394/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1261065/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1241358/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1418866/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1218300/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1176890/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:115211/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1130590/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1128019/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1107741/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1062707/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:100318/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:14785/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2089905/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2075716/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:20490/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2046042/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2043698/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1982955/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1968156/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1929684/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1907909/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1907092/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1903261/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1749141/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1670280/67‑1 (MQ=255) ccTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:2135161/67‑1 (MQ=255) ctttGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:957055/63‑1 (MQ=255) cTATGCCCTGGCATTGACGGAGCCAGGCGCAGGCTCAGACAACAACAGTGCCACTACCACTTacac < 1:1830835/66‑1 (MQ=255) ccTGGCGTTTACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:1013605/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:566971/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:794295/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:2182703/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:2151351/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:2078905/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:1403226/1‑67 (MQ=255) ccTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTaaa > 1:1302214/1‑67 (MQ=255) | GCTACGTAAAACGGCAGAGAGCACCCTGGAAACGGGTGATCCCGCCTATGCCCTGGCGTTGACGGAACCAGGTGCTGGCTCAGATAACAACAGTGCCACTACCACTTACACGCGTAAA > minE/1105198‑1105315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |