Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,142,011 T→C 100% F152L (TTC→CTC)  katE → hydroperoxidase HPII(III)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,142,0110TC100.0% 18.2 / NA 7F152L (TTC→CTC) katEhydroperoxidase HPII(III)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/0);  total (7/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGT  >  minE/1141982‑1142048
                             |                                     
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTAtttct  >  1:1436731/1‑65 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTg   >  1:1009410/1‑66 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt  >  1:1416154/1‑67 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt  >  1:2045362/1‑67 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt  >  1:2208921/1‑67 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt  >  1:438714/1‑67 (MQ=255)
aaaGCTTAAGCGATATTACCAAAGCGGATCCCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt  >  1:1741479/1‑67 (MQ=255)
                             |                                     
AAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGT  >  minE/1141982‑1142048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: