Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,142,011 | T→C | 100% | F152L (TTC→CTC) | katE → | hydroperoxidase HPII(III) |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,142,011 | 0 | T | C | 100.0% | 18.2 / NA | 7 | F152L (TTC→CTC) | katE | hydroperoxidase HPII(III) |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/0); total (7/0) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGT > minE/1141982‑1142048 | aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTAtttct > 1:1436731/1‑65 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTg > 1:1009410/1‑66 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt > 1:1416154/1‑67 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt > 1:2045362/1‑67 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt > 1:2208921/1‑67 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt > 1:438714/1‑67 (MQ=255) aaaGCTTAAGCGATATTACCAAAGCGGATCCCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGt > 1:1741479/1‑67 (MQ=255) | AAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAATCACCCCAGTATTTGT > minE/1141982‑1142048 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |