| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,142,959 | A→G | 100% | N468D (AAC→GAC) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,142,959 | 0 | A | G | 76.7% | 50.5 / 13.2 | 30 | N468D (AAC→GAC) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base G (23/0); total (30/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAA > minE/1142925‑1142991 | cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGTAACACCGCCGGGGCCGaaa > 1:1455452/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1806999/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:633132/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:586523/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:657285/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:47859/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:473359/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:345896/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:2217429/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1688795/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1647876/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1591050/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:678667/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1547638/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1442451/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:716810/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:732708/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1180730/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1067191/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaa > 1:1815373/1‑66 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaa > 1:1927239/1‑66 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaa > 1:280035/1‑66 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATGACTGGCCGCGCGAAACACCGCCGGGGCCGaa > 1:497073/1‑66 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGtaa > 1:853553/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:489033/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1590129/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:1375875/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:734955/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGaaa > 1:108069/1‑67 (MQ=255)cccGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGa > 1:1340196/1‑65 (MQ=255) | CCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAA > minE/1142925‑1142991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |